Regulatory

Part:BBa_K4307004:Design

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-30)


AttP & AttB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 24
    Illegal BsaI site found at 55
    Illegal BsaI.rc site found at 150


Design Notes

We demonstrated that the attP and attB we selected could be recognized by Integrase and trigger inversion, which laid the foundation for the design of logic gating.


Source

E.coli

References